Modification of a single tryptophan residue in human Cu, Zn-superoxide dismutase by peroxynitrite in the presence of bicarbonate
F Yamakura, T Matsumoto, T Fujimura, H Taka… - … et Biophysica Acta (BBA …, 2001 - Elsevier
Human recombinant Cu, Zn-SOD was reacted with peroxynitrite in a reaction mixture
containing 150 mM potassium phosphate buffer (pH 7.4) 25 mM sodium bicarbonate, and
0.1 mM diethylenetriamine pentaacetic acid. Disappearance of fluorescence emission at
350 nm, which could be attributed to modification of a single tryptophan residue, was
observed in the modified enzyme with a pH optimum of around 8.4. A fluorescence decrease
with the same pH optimum was also observed without sodium bicarbonate, but with less …
containing 150 mM potassium phosphate buffer (pH 7.4) 25 mM sodium bicarbonate, and
0.1 mM diethylenetriamine pentaacetic acid. Disappearance of fluorescence emission at
350 nm, which could be attributed to modification of a single tryptophan residue, was
observed in the modified enzyme with a pH optimum of around 8.4. A fluorescence decrease
with the same pH optimum was also observed without sodium bicarbonate, but with less …
