[HTML][HTML] Inducible expression of IκBα repressor mutants interferes with NF-κB activity and HIV-1 replication in Jurkat T cells
H Kwon, N Pelletier, C DeLuca, P Genin… - Journal of biological …, 1998 - ASBMB
Human immunodeficiency virus (HIV-1) utilizes the NF-κB/Rel proteins to regulate
transcription through NF-κB binding sites in the HIV-1 long terminal repeat (LTR). Normally,
NF-κB is retained in the cytoplasm by inhibitory IκB proteins; after stimulation by multiple
activators including viruses, IκBα is phosphorylated and degraded, resulting in NF-κB
release. In the present study, we examined the effect of tetracycline-inducible expression of
transdominant repressors of IκBα (TD-IκBα) on HIV-1 multiplication using stably selected …
transcription through NF-κB binding sites in the HIV-1 long terminal repeat (LTR). Normally,
NF-κB is retained in the cytoplasm by inhibitory IκB proteins; after stimulation by multiple
activators including viruses, IκBα is phosphorylated and degraded, resulting in NF-κB
release. In the present study, we examined the effect of tetracycline-inducible expression of
transdominant repressors of IκBα (TD-IκBα) on HIV-1 multiplication using stably selected …
