Serine-palmitoyl transferase activity in cultured human keratinocytes
WM Holleran, ML Williams, WN Gao, PM Elias - Journal of lipid research, 1990 - ASBMB
Sphingolipids comprise approximately 25% of the stratum corneum lipids and are
considered critical constituents of the epidermal permeability barrier. Whether sphingoid
base structures are synthesized in the epidermis or whether they are derived from circulating
or dermal sources is not known. We report here the initial characterization of serine-
palmitoyl transferase (EC 2.3. 1.50; SPT), the rate-limiting enzyme in the synthesis of
sphingolipids, from cultured human neonatal keratinocytes. Subcellular fractionation studies …
considered critical constituents of the epidermal permeability barrier. Whether sphingoid
base structures are synthesized in the epidermis or whether they are derived from circulating
or dermal sources is not known. We report here the initial characterization of serine-
palmitoyl transferase (EC 2.3. 1.50; SPT), the rate-limiting enzyme in the synthesis of
sphingolipids, from cultured human neonatal keratinocytes. Subcellular fractionation studies …
