Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro.
E Bell, B Ivarsson, C Merrill - Proceedings of the National …, 1979 - National Acad Sciences
E Bell, B Ivarsson, C Merrill
Proceedings of the National Academy of Sciences, 1979•National Acad SciencesFibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the
area of the starting gel in 24 hr. The rate of the process can be regulated by varying the
protein content of the lattice, the cell number, or the concentration of an inhibitor such as
Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left
the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The
potential uses of the system as an immunologically tolerated" tissue" for wound healing and …
area of the starting gel in 24 hr. The rate of the process can be regulated by varying the
protein content of the lattice, the cell number, or the concentration of an inhibitor such as
Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left
the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The
potential uses of the system as an immunologically tolerated" tissue" for wound healing and …
Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the concentration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immunologically tolerated "tissue" for wound healing and as a model for studying fibroblast function are discussed.
National Acad Sciences
