The glutathione content of retinal Müller (glial) cells: effect of pathological conditions
D Huster, A Reichenbach, W Reichelt - Neurochemistry international, 2000 - Elsevier
Maintenance of isolated retinal Müller (glial) cells in glutamate-free solutions over 7 h
causes a significant loss of their initial glutathione content; this loss is largely prevented by
the blockade of glutamine synthesis using methionine sulfoximine (5 mM). Anoxia does not
reduce the glutathione content of Müller cells when glucose (11 mM), glutamate and cystine
(0.1 mM each) are present. In contrast, simulation of total ischemia (ie, anoxia plus removal
of glucose) decreases the glutathione levels dramatically, even in the presence of glutamate …
causes a significant loss of their initial glutathione content; this loss is largely prevented by
the blockade of glutamine synthesis using methionine sulfoximine (5 mM). Anoxia does not
reduce the glutathione content of Müller cells when glucose (11 mM), glutamate and cystine
(0.1 mM each) are present. In contrast, simulation of total ischemia (ie, anoxia plus removal
of glucose) decreases the glutathione levels dramatically, even in the presence of glutamate …
