Accumulation of point mutations in mitochondrial DNA of aging mice
M Khaidakov, RH Heflich, MG Manjanatha… - Mutation Research …, 2003 - Elsevier
Mitochondrial DNA (mtDNA) exists in a highly genotoxic environment created by exposure to
reactive oxygen species, somewhat deficient DNA repair, and the relatively low fidelity of
polymerase gamma. Given the severity of the environment, it was anticipated that mutation
accumulation in the mtDNA of aging animals should exceed that of nuclear genes by several
orders of magnitude. We have analyzed fragments amplified from the D-loop region of
mtDNA from 2 to 22-month-old mice. The amplified 432bp fragments were cloned into …
reactive oxygen species, somewhat deficient DNA repair, and the relatively low fidelity of
polymerase gamma. Given the severity of the environment, it was anticipated that mutation
accumulation in the mtDNA of aging animals should exceed that of nuclear genes by several
orders of magnitude. We have analyzed fragments amplified from the D-loop region of
mtDNA from 2 to 22-month-old mice. The amplified 432bp fragments were cloned into …
