[HTML][HTML] Glucose-regulated glucagon secretion requires insulin receptor expression in pancreatic α-cells
J Diao, Z Asghar, CB Chan, MB Wheeler - Journal of biological chemistry, 2005 - ASBMB
The insulin receptor (IR) and its signaling appear to be essential for insulin secretion from
pancreatic β-cells. However, much less is known about the role of the IR in α-cells. To
assess the role of the IR in glucagon and insulin secretion, we engineered adeno-viruses for
high efficiency small interference RNA (siRNA)-IR expression in isolated mouse pancreatic
islets and lentiviruses for siRNA-IR expression in pancreatic α-and β-cell lines (α-TC6 and
MIN6) with specific, long term stable IR knockdown. Western blot analysis showed that these …
pancreatic β-cells. However, much less is known about the role of the IR in α-cells. To
assess the role of the IR in glucagon and insulin secretion, we engineered adeno-viruses for
high efficiency small interference RNA (siRNA)-IR expression in isolated mouse pancreatic
islets and lentiviruses for siRNA-IR expression in pancreatic α-and β-cell lines (α-TC6 and
MIN6) with specific, long term stable IR knockdown. Western blot analysis showed that these …