Methods on in situ hybridization, immunohistochemistry and β-galactosidase reporter gene detection

D Franco, PAJ de Boer… - European journal of …, 2001 - access.portico.org
Embryological studies increasingly require an integrated approach of the localization of a
molecule in the morphological context in search of the molecular mechanisms involved
during development. In our department we have become progressively experienced in the
application of molecularly based techniques to localize endogenous and transgenic RNA
and/or protein to get insight into morphogenetic processes. Here, we give a detailed
description of the use techniques for RNA localization (in situ hybridization on tissue …