Expression of the mammalian mitochondrial genome: stability of mitochondrial translation products as a function of membrane potential
C Cote, J Poirier, D Boulet - Journal of Biological Chemistry, 1989 - ASBMB
Polypeptide decay has been measured as a function of membrane potential. Mitochondrial
translation products were pulse-labeled in vitro with [35 S] methionine using isolated rat
heart mitochondria in the presence of an energy-generating system. The relative rate of
protein degradation was estimated from the specific activity (counts/min/mg of protein) of the
labeled translation products following the addition of unlabeled methionine (chase). To
modulate membrane potential, inhibitors of oxidative phosphorylation were used singly or in …
translation products were pulse-labeled in vitro with [35 S] methionine using isolated rat
heart mitochondria in the presence of an energy-generating system. The relative rate of
protein degradation was estimated from the specific activity (counts/min/mg of protein) of the
labeled translation products following the addition of unlabeled methionine (chase). To
modulate membrane potential, inhibitors of oxidative phosphorylation were used singly or in …
