A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.
Proceedings of the National Academy of Sciences, 1990•National Acad Sciences
The ability of enteropathogenic Escherichia coli (EPEC) to form attaching and effacing
intestinal lesions is a major characteristic of EPEC pathogenesis. Using TnphoA
mutagenesis we have identified a chromosomal gene (eae, for E. coli attaching and
effacing) that is necessary for this activity. A DNA probe derived from this gene hybridizes to
100% of E. coli of EPEC serogroups that demonstrate attaching and effacing activity on
tissue culture cells as well as other pathogenic E. coli that produce attaching and effacing …
intestinal lesions is a major characteristic of EPEC pathogenesis. Using TnphoA
mutagenesis we have identified a chromosomal gene (eae, for E. coli attaching and
effacing) that is necessary for this activity. A DNA probe derived from this gene hybridizes to
100% of E. coli of EPEC serogroups that demonstrate attaching and effacing activity on
tissue culture cells as well as other pathogenic E. coli that produce attaching and effacing …
The ability of enteropathogenic Escherichia coli (EPEC) to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis. Using TnphoA mutagenesis we have identified a chromosomal gene (eae, for E. coli attaching and effacing) that is necessary for this activity. A DNA probe derived from this gene hybridizes to 100% of E. coli of EPEC serogroups that demonstrate attaching and effacing activity on tissue culture cells as well as other pathogenic E. coli that produce attaching and effacing intestinal lesions, such as RDEC-1 (an EPEC of weanling rabbits) and enterohemorrhagic E. coli. The predicted amino acid sequence derived from the nucleotide sequence of eae shows significant homology to that of the invasin of Yersinia pseudotuberculosis.
National Acad Sciences
