[HTML][HTML] Intracellular delivery of phosphatidylinositol (3, 4, 5)-trisphosphate causes incorporation of glucose transporter 4 into the plasma membrane of muscle and fat …
G Sweeney, RR Garg, RB Ceddia, D Li, M Ishiki… - Journal of Biological …, 2004 - ASBMB
Insulin stimulates glucose uptake into muscle and fat cells by translocating glucose
transporter 4 (GLUT4) to the cell surface, with input from phosphatidylinositol (PI) 3-kinase
and its downstream effector Akt/protein kinase B. Whether PI 3, 4, 5-trisphosphate (PI (3, 4,
5) P 3) suffices to produce GLUT4 translocation is unknown. We used two strategies to
deliver PI (3, 4, 5) P 3 intracellularly and two insulin-sensitive cell lines to examine Akt
activation and GLUT4 translocation. In 3T3-L1 adipocytes, the acetoxymethyl ester of PI (3 …
transporter 4 (GLUT4) to the cell surface, with input from phosphatidylinositol (PI) 3-kinase
and its downstream effector Akt/protein kinase B. Whether PI 3, 4, 5-trisphosphate (PI (3, 4,
5) P 3) suffices to produce GLUT4 translocation is unknown. We used two strategies to
deliver PI (3, 4, 5) P 3 intracellularly and two insulin-sensitive cell lines to examine Akt
activation and GLUT4 translocation. In 3T3-L1 adipocytes, the acetoxymethyl ester of PI (3 …