[HTML][HTML] Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization

ST Koury, MC Bondurant, MJ Koury - Blood, 1988 - Elsevier
ST Koury, MC Bondurant, MJ Koury
Blood, 1988Elsevier
In situ hybridization was used to localize the cells that produce erythropoietin (EP) in anemic
murine kidneys. Kidneys from anemic and nonanemic mice were fixed and processed for
paraffin embedding. Sections were hybridized with a 35 S-labeled RNA probe
complementary to mRNA coding for EP. An uncommon, but specific type of cell was
intensely labeled in the cortices of anemic kidneys. The labeled cells were clearly
nonglomerular and nontubu-lar. Their location outside of the tubular basement membrane …
In situ hybridization was used to localize the cells that produce erythropoietin (EP) in anemic murine kidneys. Kidneys from anemic and nonanemic mice were fixed and processed for paraffin embedding. Sections were hybridized with a 35S-labeled RNA probe complementary to mRNA coding for EP. An uncommon, but specific type of cell was intensely labeled in the cortices of anemic kidneys. The labeled cells were clearly nonglomerular and nontubu-lar. Their location outside of the tubular basement membrane was consistent with that of a subset of interstitial cells or capillary endothelial cells.1988 by Grune & Stratton, Inc. 0006-4971/88/7102-0038$3.00/0
Elsevier