Chemokine and cytokine levels in osteoarthritis and rheumatoid arthritis synovial fluid

U Hampel, S Sesselmann, P Iserovich, S Sel… - Journal of …, 2013 - Elsevier
To develop a method of the assay of chemokine and cytokine signaling in synovial fluid from
patients suffering from osteoarthritis (OA) or rheumatoid arthritis (RA) and evaluate the effect
of heterophilic antibodies on the reliability of the data. 21 synovial fluid samples from OA and
16 synovial fluid samples from RA patients were analyzed using a unique 2 step dot
sandwich ELISA based micro-well protein array designed to detect heterophilic antibody
signaling in the presence or absence of an effective heterophilic blocking reagent with …