Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide-and proteoglycan-rich sources.

P Chomczynski, K Mackey - Biotechniques, 1995 - europepmc.org
P Chomczynski, K Mackey
Biotechniques, 1995europepmc.org
A modification of the TRI Reagent procedure has been elaborated for isolation of RNA from
polysaccharide-and proteoglycan-rich material. In the modified procedure, RNA is
precipitated from the aqueous phase by the combined action of isopropanol and a high-salt
concentration. Under these conditions, RNA is effectively precipitated while contaminating
polysaccharides and proteoglycans remain in the soluble form. The modified precipitation
does not prolong or increase the complexity of the TRI Reagent procedure. The new …
A modification of the TRI Reagent procedure has been elaborated for isolation of RNA from polysaccharide-and proteoglycan-rich material. In the modified procedure, RNA is precipitated from the aqueous phase by the combined action of isopropanol and a high-salt concentration. Under these conditions, RNA is effectively precipitated while contaminating polysaccharides and proteoglycans remain in the soluble form. The modified precipitation does not prolong or increase the complexity of the TRI Reagent procedure. The new procedure was tested by isolation of RNA from polysaccharide-and proteoglycan-rich tissues such as rat liver and aorta.
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