Nonalcoholic steatohepatitis (NASH) is a lipotoxic disease wherein activation of
endoplasmic reticulum (ER) stress response and macrophage-mediated hepatic
inflammation are key pathogenic features. However, the lipid mediators linking these two
observations remain elusive. We postulated that ER stress-regulated release of pro-
inflammatory extracellular vesicles (EVs) from lipotoxic hepatocytes may be this link. EVs
were isolated from cell culture supernatants of hepatocytes treated with palmitate (PA) to …