Generation of new Notch2 mutant alleles

B McCright, J Lozier, T Gridley - Genesis, 2006 - Wiley Online Library
B McCright, J Lozier, T Gridley
Genesis, 2006Wiley Online Library
The Notch signaling pathway is an evolutionarily conserved intercellular signaling
mechanism, and mutations in its components disrupt embryonic development in many
organisms and cause inherited diseases in humans. We previously described construction
and analysis of a hypomorphic allele of the Notch2 gene. Homozygosity for this allele leads
to embryonic and perinatal lethality due to cardiovascular and kidney defects. We report
here novel Notch2 mutant alleles generated by gene targeting in embryonic stem cells …
Abstract
The Notch signaling pathway is an evolutionarily conserved intercellular signaling mechanism, and mutations in its components disrupt embryonic development in many organisms and cause inherited diseases in humans. We previously described construction and analysis of a hypomorphic allele of the Notch2 gene. Homozygosity for this allele leads to embryonic and perinatal lethality due to cardiovascular and kidney defects. We report here novel Notch2 mutant alleles generated by gene targeting in embryonic stem cells, including a conditional null allele in which exon 3 of the Notch2 gene is flanked by loxP sequences. These new Notch2 mutant alleles expand the set of tools available for studying the myriad roles of the Notch pathway during mammalian development and will enable analysis of Notch2 function at additional stages of embryogenesis and in adult mice. genesis 44:29–33, 2006. © 2006 Wiley‐Liss, Inc.
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