[HTML][HTML] Immune cells control skin lymphatic electrolyte homeostasis and blood pressure

H Wiig, A Schröder, W Neuhofer… - The Journal of …, 2013 - Am Soc Clin Investig
H Wiig, A Schröder, W Neuhofer, J Jantsch, C Kopp, TV Karlsen, M Boschmann, J Goss…
The Journal of clinical investigation, 2013Am Soc Clin Investig
The skin interstitium sequesters excess Na+ and Cl–in salt-sensitive hypertension.
Mononuclear phagocyte system (MPS) cells are recruited to the skin, sense the hypertonic
electrolyte accumulation in skin, and activate the tonicity-responsive enhancer-binding
protein (TONEBP, also known as NFAT5) to initiate expression and secretion of VEGFC,
which enhances electrolyte clearance via cutaneous lymph vessels and increases eNOS
expression in blood vessels. It is unclear whether this local MPS response to osmotic stress …
The skin interstitium sequesters excess Na+ and Cl in salt-sensitive hypertension. Mononuclear phagocyte system (MPS) cells are recruited to the skin, sense the hypertonic electrolyte accumulation in skin, and activate the tonicity-responsive enhancer-binding protein (TONEBP, also known as NFAT5) to initiate expression and secretion of VEGFC, which enhances electrolyte clearance via cutaneous lymph vessels and increases eNOS expression in blood vessels. It is unclear whether this local MPS response to osmotic stress is important to systemic blood pressure control. Herein, we show that deletion of TonEBP in mouse MPS cells prevents the VEGFC response to a high-salt diet (HSD) and increases blood pressure. Additionally, an antibody that blocks the lymph-endothelial VEGFC receptor, VEGFR3, selectively inhibited MPS-driven increases in cutaneous lymphatic capillary density, led to skin Cl accumulation, and induced salt-sensitive hypertension. Mice overexpressing soluble VEGFR3 in epidermal keratinocytes exhibited hypoplastic cutaneous lymph capillaries and increased Na+, Cl, and water retention in skin and salt-sensitive hypertension. Further, we found that HSD elevated skin osmolality above plasma levels. These results suggest that the skin contains a hypertonic interstitial fluid compartment in which MPS cells exert homeostatic and blood pressure–regulatory control by local organization of interstitial electrolyte clearance via TONEBP and VEGFC/VEGFR3–mediated modification of cutaneous lymphatic capillary function.
The Journal of Clinical Investigation