Isolation and characterization of neutrophil-derived microparticles for functional studies
A Finkielsztein, L Mascarenhas, V Butin-Israeli… - JoVE (Journal of …, 2018 - jove.com
Polymorphonuclear neutrophil-derived microparticles (PMN)-MPs) are lipid bilayer,
spherical microvesicles with sizes ranging from 50–1,000 nm in diameter. MPs are a newly
evolving, important part of cell-to-cell communication and signaling machinery. Because of
their size and the nature of their release, until recently MP existence was overlooked.
However, with improved technology and analytical methods their function in health and
disease is now emerging. The protocols presented here are aimed at isolating and …
spherical microvesicles with sizes ranging from 50–1,000 nm in diameter. MPs are a newly
evolving, important part of cell-to-cell communication and signaling machinery. Because of
their size and the nature of their release, until recently MP existence was overlooked.
However, with improved technology and analytical methods their function in health and
disease is now emerging. The protocols presented here are aimed at isolating and …