Highly efficient mouse genome editing by CRISPR ribonucleoprotein electroporation of zygotes
S Chen, B Lee, AYF Lee, AJ Modzelewski… - Journal of Biological …, 2016 - ASBMB
The CRISPR/Cas9 system has been employed to efficiently edit the genomes of diverse
model organisms. CRISPR-mediated mouse genome editing is typically accomplished by
microinjection of Cas9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate
modified animals in one step. However, microinjection is a technically demanding, labor-
intensive, and costly procedure with poor embryo viability. Here, we describe a simple and
economic electroporation-based strategy to deliver Cas9/sgRNA ribonucleoproteins into …
model organisms. CRISPR-mediated mouse genome editing is typically accomplished by
microinjection of Cas9 DNA/RNA and single guide RNA (sgRNA) into zygotes to generate
modified animals in one step. However, microinjection is a technically demanding, labor-
intensive, and costly procedure with poor embryo viability. Here, we describe a simple and
economic electroporation-based strategy to deliver Cas9/sgRNA ribonucleoproteins into …
