[HTML][HTML] Maintenance of HSC by Wnt5a secreting AGM-derived stromal cell line
SM Buckley, F Ulloa-Montoya, D Abts… - Experimental …, 2011 - Elsevier
OBJECTIVE: The microenvironment wherein hematopoietic stem cells (HSC) reside
orchestrates HSC self-renewal vs. differentiation decisions. Stromal cells derived from
ontogenically divergent hematopoietic microenvironments can support HSC in vitro and
have been used to decipher factors that influence HSC fate decisions. Employing stromal
cell lines derived from the aorta-gonad-mesonephros and embryonic liver, we aim to identify
secreted factors that maintain/expand HSC in vitro. MATERIALS AND METHODS: We …
orchestrates HSC self-renewal vs. differentiation decisions. Stromal cells derived from
ontogenically divergent hematopoietic microenvironments can support HSC in vitro and
have been used to decipher factors that influence HSC fate decisions. Employing stromal
cell lines derived from the aorta-gonad-mesonephros and embryonic liver, we aim to identify
secreted factors that maintain/expand HSC in vitro. MATERIALS AND METHODS: We …
