[HTML][HTML] Lipopolysaccharide down-regulates Sp1 binding activity by promoting Sp1 protein dephosphorylation and degradation
X Ye, SF Liu - Journal of Biological Chemistry, 2002 - ASBMB
We examined the in vivo effect of lipopolysaccharide (LPS) on Sp1 (promoter-selective
transcription factor 1) DNA binding activity and studied the mechanisms involved in mouse
lungs. The Sp1 DNA complex displayed a major band composed of Sp1, Sp2, and Sp3
trimer and a minor band composed of Sp3 homodimer. Compared with control, nuclear
proteins from lungs challenged with LPS for 60, 90, 120, 150, 180, and 240 min,
respectively, showed a markedly reduced Sp1 binding activity. Down-regulation of Sp1 …
transcription factor 1) DNA binding activity and studied the mechanisms involved in mouse
lungs. The Sp1 DNA complex displayed a major band composed of Sp1, Sp2, and Sp3
trimer and a minor band composed of Sp3 homodimer. Compared with control, nuclear
proteins from lungs challenged with LPS for 60, 90, 120, 150, 180, and 240 min,
respectively, showed a markedly reduced Sp1 binding activity. Down-regulation of Sp1 …
