Ketogenesis in isolated rat liver mitochondria. III. Relationship with the rate of β-oxidation
M Lopes-Cardozo, SG Van den Bergh - Biochimica et Biophysica Acta (BBA …, 1974 - Elsevier
M Lopes-Cardozo, SG Van den Bergh
Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1974•ElsevierThe synthesis of ketone bodies has been studied as a function of the rate of acetyl-CoA
production during the oxidation of different fatty acids and of pyruvate. In the presence of
hexokinase plus glucose and a low concentration of malate, the Krebs cycle has a fixed
capacity which is independent of the rate of acetyl-CoA supply. When the rate of acetyl-CoA
production increases beyond this capacity, acetyl-CoA is converted to the synthesis of
acetoacetate. Under all conditions tested the rate of ketogenesis and the ratio of acetyl-CoA …
production during the oxidation of different fatty acids and of pyruvate. In the presence of
hexokinase plus glucose and a low concentration of malate, the Krebs cycle has a fixed
capacity which is independent of the rate of acetyl-CoA supply. When the rate of acetyl-CoA
production increases beyond this capacity, acetyl-CoA is converted to the synthesis of
acetoacetate. Under all conditions tested the rate of ketogenesis and the ratio of acetyl-CoA …
Abstract
The synthesis of ketone bodies has been studied as a function of the rate of acetyl-CoA production during the oxidation of different fatty acids and of pyruvate. In the presence of hexokinase plus glucose and a low concentration of malate, the Krebs cycle has a fixed capacity which is independent of the rate of acetyl-CoA supply. When the rate of acetyl-CoA production increases beyond this capacity, acetyl-CoA is converted to the synthesis of acetoacetate. Under all conditions tested the rate of ketogenesis and the ratio of acetyl-CoA over free CoASH were positively correlated.
The relevance of our results for the control of ketogenesis in vivo is discussed.
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