[HTML][HTML] Identification of the paired basic convertases implicated in HIV gp160 processing based on in vitro assays and expression in CD4+ cell lines
E Decroly, S Wouters, C Di Bello, C Lazure… - Journal of Biological …, 1996 - ASBMB
The human immunodeficiency virus HIV envelope glycoprotein gp160 is synthesized as an
inactive precursor, which is processed into its fusiogenic form gp120/gp41 by host cell
proteinases during its intracellular trafficking. Kexin/subtilisin-related endoproteases have
been proposed to be enzyme candidates for this maturation process. In the present study, 1)
we examined the ability of partially purified precursor convertases and their isoforms to
cleave gp160 in vitro. The data demonstrate that all the convertases tested specifically …
inactive precursor, which is processed into its fusiogenic form gp120/gp41 by host cell
proteinases during its intracellular trafficking. Kexin/subtilisin-related endoproteases have
been proposed to be enzyme candidates for this maturation process. In the present study, 1)
we examined the ability of partially purified precursor convertases and their isoforms to
cleave gp160 in vitro. The data demonstrate that all the convertases tested specifically …