Mouse genome editing using the CRISPR/Cas system
DW Harms, RM Quadros, D Seruggia… - Current protocols in …, 2014 - Wiley Online Library
Current protocols in human genetics, 2014•Wiley Online Library
The availability of techniques to create desired genetic mutations has enabled the laboratory
mouse as an extensively used model organism in biomedical research including human
genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system.
Specifically, this system allows editing of the mouse genome much more quickly than the
previously used techniques, and, more importantly, multiple mutations can be created in a
single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and …
mouse as an extensively used model organism in biomedical research including human
genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system.
Specifically, this system allows editing of the mouse genome much more quickly than the
previously used techniques, and, more importantly, multiple mutations can be created in a
single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and …
Abstract
The availability of techniques to create desired genetic mutations has enabled the laboratory mouse as an extensively used model organism in biomedical research including human genetics. A new addition to this existing technical repertoire is the CRISPR/Cas system. Specifically, this system allows editing of the mouse genome much more quickly than the previously used techniques, and, more importantly, multiple mutations can be created in a single experiment. Here we provide protocols for preparation of CRISPR/Cas reagents and microinjection into one‐cell mouse embryos to create knockout or knock‐in mouse models. Curr. Protoc. Hum. Genet. 83:15.7.1‐15.7.27. © 2014 by John Wiley & Sons, Inc.
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